Studies On Effect of Various Agro-Industrial Waste on Milk-Clotting Activity Extracted from Aspergillus Tamarii

Abstract

Effect of five agro-industrial waste and casein on enzyme (protease) activity of Aspergillus tamari were studied using solid state fermentation; protease constitute one of the most important groups of global industrial enzymes, screening substrates and organisms for better production can add to country’s economy. The six substrates were screened as media for the production of protease from A. tamari by using 5.0g of each substrate and 1 ml spore suspension (10⁶ spores/ml) of A. tamari; crude enzymes was filtered using Whatman No. 1 filter paper, purified by ammonium sulphate precipitation, milk clotting and protease activity analysis were conducted for the enzymes extracted from each substrate. Results indicated that supernatant enzymes from banana peel powder had the highest milk-clotting activity of 351.2 SU/mL within 382.7 seconds. While RB supernatant enzyme had the lowest MCA. The pellet enzymes extracted from fermentation of the substrate with A. tamari showed low milk-clotting activity, but still pellet enzyme from Bpp had high MCA of 387.3 SU/mL at 310 seconds. However protease activity (PA) of the enzyme was high with the supernatant of millet bran (0.40±0.01) A. tamari had shown a high milk-clotting activity when treated with Bpp and and high PA on treatment with MB, further studies on production of protease from A. tamari with the combination of the two substrates is necessary to determine their implication when combined.